I don't have one, but I would think it would probably just be the 5-page story at the end of issue 1...? This was originally planned to be the first five pages of issue 1, but Kurtis rewrote the beginning, and this was then added as a supplemental short story at the end of issue 1.
I don't see this 5 page story you're talking about in my copies of #1. My copies of #1 have a five page preview for Drumhellar at the end.
I assume the 5 page story you're referring to that was originally supposed to be the opening of the comic is the 5-page Hannah story posted online as a preview, which I linked to earlier. But this story is not printed in the copies of #1 I have.
The WN story is the Hannah/RQ story but the final panel does have all the Rat Queens. So does that make it a 1st appearance if it's not printed anywhere else???
I say YES (thumbs u